Recipe

TEVCB Buffer

Reagent Quantity (for 50 mL) Final concentration
Tris-Cl (1 M, pH 8.0) 0.5 mL 10 mM
NaCl (5 M) 1.5 mL 150 mM
NP-40 (10%) 0.5 mL 0.1%
EDTA (0.5 M) 50 µL 0.5 mM
DTT (1 M) 25 µL 1.0 mM

  • Adjust to a final volume of 50 mL with H2O. Add the DTT just prior to use.

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    1. doi:10.1101/pdb.rec12434 Cold Spring Harb Protoc 2011: pdb.rec12434- © 2011 Cold Spring Harbor Laboratory Press
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