CEB Buffer
| Reagent | Quantity (for 10 mL) | Final concentration |
|---|---|---|
| Tris-HCl (1 M, pH 8.0) | 0.1 mL | 10 mM |
| NaCl (5 M) | 0.3 mL | 150 mM |
| Imidazole (1 M) | 10 µL | 1 mM |
| Magnesium acetate (1 M) | 10 µL | 1 mM |
| EGTA (0.5 M) | 400 µL | 20 mM |
| β-Mercaptoethanol (β-ME) | ||
| (14.3 M) | 7 µL | 10 mM |
Adjust to a final volume of 10 mL with H2O. Add the β-ME to the CEB buffer just prior to use.
- © 2011 Cold Spring Harbor Laboratory Press
