Multimodality imaging of mESC survival, proliferation, and migration after cardiac delivery with a triple-fusion reporter gene. (A) Schema of the triple-fusion reporter gene. (B) Fluorescence-activated cell sorting (FACS) histograms of mESCs at 48 h after transduction with plasmid lipofectamine, electroporation, and lentivirus carrying the triple-fusion reporter gene. (C) Noninvasive imaging of transplanted mESCs with BLI and PET. Radiance is given in photons/sec per cm²/steradian. (Adapted from Cao et al. 2006 and reprinted with permission from Wolters Kluwer Health © 2006.)