Topic Introduction

Maintaining Live Cells and Tissue Slices in the Imaging Setup

  1. Dana Kurpius
Adapted from Imaging in Neuroscience (ed. Helmchen and Konnerth). CSHL Press, Cold Spring Harbor, NY, USA, 2011.

INTRODUCTION

Live imaging provides exciting opportunities to study dynamic cellular events as they occur in real time. However, such experimental procedures present several challenges. This article discusses some of the major considerations relating to the maintenance of live biological samples during ex vivo imaging and presents some relatively simple low-cost solutions for keeping samples healthy.

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  1. doi:10.1101/pdb.top105 Cold Spring Harb Protoc 2011: pdb.top105- © 2011 Cold Spring Harbor Laboratory Press
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