Isolation of Human Capillary Endothelial Cells from Abdominal Adipose Tissue
Adapted from Cells (ed. Spector et al.). CSHL Press, Cold Spring Harbor, NY, USA, 1998.INTRODUCTION
This protocol outlines a technique to isolate human capillary endothelial cells from human abdominal wall adipose tissue. The relative lack of cell-type complexity within fat tissue makes it an attractive source of endothelial cells. In addition, collaboration with a local plastic surgeon can yield significant quantities of subcutaneous adipose tissue from liposuction without the need to be on call for cadaveric or post-surgical samples.
- © 2011 Cold Spring Harbor Laboratory Press
