Methylcellulose overlay

Add 25 g of methylcellulose to 100 mL of PBS for HSV at pH 7.5 in a 500-mL sterile bottle containing a stir bar and autoclave the bottle on liquid cycle (45 min). After the solution cools, add 350 mL of DMEM supplemented with nonessential amino acids, 100 units/mL penicillin G, 100 µg/mL streptomycin sulfate, and 2 mM glutamine. Mix well and place the bottle overnight on a stir plate at 4°C. Once the methylcellulose has entered solution, add 50 mL of fetal bovine serum (FBS).

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