Base buffer (2×)
50 mM HEPES (pH 8.0)
100 mM L-glutamic acid potassium salt monohydrate
0.2% Triton X-100
16 mM magnesium acetate tetrahydrate
20% glycerol
Filter-sterilize with a 0.2-µm Nalgene filter and store at 4°C.
- © 2011 Cold Spring Harbor Laboratory Press
