Topic Introduction

Two-Photon Mapping of Neural Circuits

  1. Rafael Yuste
Adapted from Imaging in Neuroscience: A Laboratory Manual (eds. Helmchen and Konnerth). CSHL Press, Cold Spring Harbor, NY, USA, 2011.

INTRODUCTION

For the study of neural circuits, it would be ideal to be able to discern in a living preparation the interconnections among neurons. Working toward that aim, we have developed a method, using two-photon uncaging of glutamate in brain slices, by which we can detect neurons that are presynaptic to any particular cell. The method, described here, has single-cell resolution and can be applied systematically and quickly to test entire populations of neurons, making it possible to perform online functional analysis of connectivity.

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  1. doi:10.1101/pdb.top111 Cold Spring Harb Protoc 2011: pdb.top111- © 2011 Cold Spring Harbor Laboratory Press
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