Two-Photon Mapping of Neural Circuits
Adapted from Imaging in Neuroscience: A Laboratory Manual (eds. Helmchen and Konnerth). CSHL Press, Cold Spring Harbor, NY, USA, 2011.INTRODUCTION
For the study of neural circuits, it would be ideal to be able to discern in a living preparation the interconnections among neurons. Working toward that aim, we have developed a method, using two-photon uncaging of glutamate in brain slices, by which we can detect neurons that are presynaptic to any particular cell. The method, described here, has single-cell resolution and can be applied systematically and quickly to test entire populations of neurons, making it possible to perform online functional analysis of connectivity.
- © 2011 Cold Spring Harbor Laboratory Press
