Dissection and Staining of Drosophila Optic Lobes at Different Stages of Development
Adapted from Drosophila Neurobiology (ed. Zhang et al.). CSHL Press, Cold Spring Harbor, NY, USA, 2010.INTRODUCTION
The Drosophila visual system is composed of the retina and the optic lobes, which are the ganglia where photoreceptors project and initial processing of visual inputs occur. This protocol outlines procedures for dissecting the optic lobes from Drosophila larvae, pupae, and adults. It also describes methods for visualizing the anatomy of brain neural circuits by staining with fluorescent secondary antibodies and primary antibodies specific for various neuronal populations and architectural features.
- © 2011 Cold Spring Harbor Laboratory Press
