Recipe

Danieau’s solution (30×)

Reagent Amount per 1 L Concentration
NaCl 101.7 g 1740 mM
KCl 1.56 g 21 mM
MgSO4•7H2O 2.96 g 12 mM
Ca(NO3)2 4.25 g 18 mM
HEPES buffer 35.75 g 150 mM
Add water to 1 L and stir until dissolved. Store at 4°C. The pH is 7.6.

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  1. doi:10.1101/pdb.rec12467 Cold Spring Harb Protoc 2011: pdb.rec12467- © 2011 Cold Spring Harbor Laboratory Press
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