Danieau’s solution (30×)
| Reagent | Amount per 1 L | Concentration |
|---|---|---|
| NaCl | 101.7 g | 1740 mM |
| KCl | 1.56 g | 21 mM |
| MgSO4•7H2O | 2.96 g | 12 mM |
| Ca(NO3)2 | 4.25 g | 18 mM |
| HEPES buffer | 35.75 g | 150 mM |
| Add water to 1 L and stir until dissolved. Store at 4°C. The pH is 7.6. | ||
- © 2011 Cold Spring Harbor Laboratory Press
