Protocol

On-Membrane Proteolytic Digestion of Electroblotted Proteins

  1. Richard J. Simspon
Adapted from Proteins and Proteomics by Richard J. Simpson. CSHL Press, Cold Spring Harbor, NY, 2003.

INTRODUCTION

There are two main approaches for digesting proteins to generate peptide maps for gel-separated proteins: (1) protein blotting onto immobilizing membranes, followed by on-membrane digestion, and (2) digesting proteins directly in the gel matrix and extracting the peptides. In this protocol, a method for performing on-membrane protein digestion is provided.

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  1. doi:10.1101/pdb.prot4589 Cold Spring Harb Protoc 2011: pdb.prot4589- © 2011 Cold Spring Harbor Laboratory Press
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