Immunolabeling of Drosophila Embryos and Tissues
Adapted from Basic Methods in Microscopy (ed. Spector and Goldman). CSHL Press, Cold Spring Harbor, NY, USA, 2006.INTRODUCTION
The fruit fly Drosophila melanogaster has long been used to study the genetic factors involved in development. The ability to localize molecules within an organism that allow genetic manipulation can be quite useful. This article provides a standard method for preparing various Drosophila tissues for analysis by immunofluorescence microscopy. Specific methods are also presented for the handling of embryos, because these are used frequently and require special consideration.
- © 2011 Cold Spring Harbor Laboratory Press
