Protocol

Single-Particle Virus Tracking

Adapted from Single-Molecule Techniques (ed. Selvin and Ha). CSHL Press, Cold Spring Harbor, NY, USA, 2008.

INTRODUCTION

Real-time, live-cell imaging techniques and single-particle tracking algorithms can be used to follow individual virus particles as they infect cells. This protocol describes the use of one or more fluorescent markers to perform single-particle virus-tracking experiments.

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