Paraformaldehyde Fixation of Drosophila Testes
Adapted from Drosophila Protocols (ed. Sullivan et al.). CSHL Press, Cold Spring Harbor, NY, USA, 2000.Abstract
This protocol describes paraformaldehyde fixation of Drosophila testes. The procedure preserves F-actin-containing structures because it does not involve the use of methanol. Thus, it is particularly suitable for contractile ring and fusome visualization. However, it does not preserve cell morphology and microtubules as efficiently as methanol–acetone fixation and does not permit clear detection of the Y loops.
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