Isolation of DNA from Red Blood Cells in Xenopus
Adapted from Early Development of Xenopus laevis (ed. Sive et al.). CSHL Press, Cold Spring Harbor, NY, USA, 2000.Abstract
The amphibian Xenopus laevis is an important model organism that is particularly valuable for studies of early vertebrate development. Genomic DNA constitutes the total genetic information of an organism and it is used for Southern blotting, for determining gene structure, and for detecting the presence or absence of genes of interest. Genomic DNA can be extracted from Xenopus red blood cells, which are unlike the mammalian equivalent in that they contain nuclei. This article describes a protocol for the isolation of genomic DNA from frog red blood cells.
- © 2012 Cold Spring Harbor Laboratory Press
