Recipe

Nuclei Freezing Buffer

Reagent Final concentration
Glycerol 50%
NaCl 0.15 m
MgCl2 5 mm
Tris-HCl (pH 8) 10 mm
EDTA 0.1 mm
Dithiothreitol (DTT) 1 mm
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  1. doi:10.1101/pdb.rec067751 Cold Spring Harb Protoc 2012: pdb.rec067751- © 2012 Cold Spring Harbor Laboratory Press
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