X-gal Stain for FIV

Potassium ferricyanide

Potassium ferrocyanide

MgCl₂

X-gal

Dimethyl sulfoxide (DMSO)

Phosphate-buffered saline (PBS; tissue-culture grade)

• 1. Prepare buffer stock (10×) of 50 mm potassium ferricyanide, 50 mm potassium ferrocyanide, and 20 mm MgCl₂ in H₂O.

• 2. Prepare X-gal stock (40×) of 40 mg/mL X-gal in DMSO.

• 3. Prepare the final solution, which is 1× buffer and 1 mg/mL X-gal diluted in PBS. Store at –80°C in small aliquots.

• © 2012 Cold Spring Harbor Laboratory Press