Protocol

Mammary Fat Pad Chamber Preparation in Mice

Adapted from Live Cell Imaging, 2nd edition (ed. Goldman et al.). CSHL Press, Cold Spring Harbor, NY, USA, 2010.

Abstract

This protocol describes a procedure to implant mammary fat pad chambers in mice. These chambers can then be used for studying breast cancer. In comparison to the classical mammary fat pad flap model, the chamber described here has the advantage of allowing multiple observation time points in the same animal with simple anesthesia.

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