Master Binding Mix
| Reagent | Quantity (for 1 mL) | Final concentration |
|---|---|---|
| Tris (1 m, pH 7.6) | 20 µL | 20 mm |
| NaCl or KCl (1 m) | 20–200 µL | 20–200 mm |
| Dithiothreitol (DTT; 1 m) | 1 µL | 1 mm |
| Bovine serum albumin (BSA; 20 mg/mL) | 2.5 µL | 50 µg/mL |
| Glycerol (50%, w/v) | 200 µL | 10% |
| H2O | to 1 mL | – |
Store for 6 mo at –20°C. Add MgCl2 or magnesium acetate to 1–5 mm if needed.
- © 2012 Cold Spring Harbor Laboratory Press
