Wash Buffer for Filter-Binding Assay
| Reagent | Quantity (for 100 mL) | Final concentration |
|---|---|---|
| Tris (1 m, pH 7.6) | 2 mL | 20 mm |
| NaCl or KCl (1 m) | 2–20 mL | 20–200 mm |
| H2O | to 100 mL |
Store indefinitely at 4°C. Add 1 mm dithiothreitol (DTT) just before use. Adjust salt concentrations as needed and add detergent as needed to wash buffer.
- © 2012 Cold Spring Harbor Laboratory Press
