Lysis Buffer for Yeast RNA
| Reagent | Quantity (for 100 mL) | Final concentration |
|---|---|---|
| Tris–HCl (1 m, pH 6.8) | 5 mL | 50 mm |
| NaCl (1 m) | 10 mL | 100 mm |
| EDTA (0.5 m, pH 8.0) | 2 mL | 10 mm |
| ddH2O | to 100 mL | – |
Buffer can be prepared well in advance; store indefinitely at room temperature.
- © 2012 Cold Spring Harbor Laboratory Press
