AE Buffer for Yeast RNA
| Reagent | Quantity (for 10 mL) | Final concentration |
|---|---|---|
| Sodium acetate (NaOAc; 3 m, pH 5.2) | 0.167 mL | 50 mm |
| EDTA (0.5 m, pH 8.0) | 0.2 mL | 10 mm |
| ddH2O | to 10 mL | – |
Prepare fresh; store for no longer than 1 wk at room temperature.
- © 2012 Cold Spring Harbor Laboratory Press
