Reverse Transcriptase Mix Stock
| Reagent | Quantity (for 60 µL) | Final concentration |
|---|---|---|
| First-strand buffer (5×; Invitrogen) | 12 µL | 2.5 mm |
| dNTP (10 mm each) | 15 µL | 2.5 mm |
| Dithiothreitol (DTT; 0.1 m) | 3 µL | 5 mm |
| Milli-Q H2O | 30 µL | – |
- © 2012 Cold Spring Harbor Laboratory Press
