Table 1.

Characteristics of sample microendoscope probes

Microendoscope type Diameter (mm) Length (mm) Usable field of view (µm) Lateral magnification) (×) Two-photon lateral resolution (FWHM, µm) NA
Doublet (0.75/0.21 pitch) 1.0 20.6 275 2.52 0.9 0.49
Doublet (1.25/0.19 pitch) 0.5 16.4 130 2.48 1.0 0.48
Doublet (1.75/0.16 pitch) 0.35 15.8 75 2.69 1.2 0.45
Singlet (0.46 pitch) 1.0 4.4 700 0.97 0.9 0.49
Singlet (0.94 pitch) 0.5 4.3 350 0.92 1.0 0.47
GRIN/plano-convex doublet (BK7 plano-convex lens) 1.0 3.7 120 1.41 0.8 0.65
GRIN/plano-convex doublet (LaSFN9 plano-convex lens) 1.0 4.0 75 1.86 0.6 0.82
  • To facilitate comparisons between parameter values, each microendoscope listed has an optical working distance of 250 µm. The lateral resolution of two-photon imaging is given for 920-nm illumination.

  • FWHM, full width at half-maximum; NA, numerical aperture; GRIN, gradient refractive index.

This Article

  1. Cold Spring Harb Protoc 2012: pdb.top071464-