SDS Extraction Buffer
| Reagent | Quantity (for 500 mL) |
Final concentration |
|---|---|---|
| Tris–HCl (1 m, pH 7.5) | 10 mL | 20 mm |
| EDTA (0.5 m, pH 8.0) | 1 mL | 1 mm |
| SDS (10% w/v) | 25 mL | 0.5% (w/v) |
| H2O | 464 mL | – |
| Store indefinitely at room temperature. | ||
- © 2012 Cold Spring Harbor Laboratory Press
