Transcription Buffer for RNA Polymerase (5×)
| Reagent | Quantity (for 10 mL) | Final concentration |
|---|---|---|
| Tris–HCl (1 m, pH 8.0) | 2 mL | 200 mm |
| MgCl2 (1 m) | 400 µL | 40 mm |
| NaCl (5 m) | 0.5 mL | 250 mm |
| Spermidine (100 mm) | 1 mL | 10 mm |
| H2O | 6.1 mL | – |
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Store for up to 1 yr at –20°C.
- © 2012 Cold Spring Harbor Laboratory Press
