Recipe

PXL Buffer

PBS for CLIP

0.1% SDS

0.5% deoxycholate

0.5% NP-40

Protease inhibitor cocktail

Some antibodies do poorly with deoxycholate; if so, omit it.

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  1. doi:10.1101/pdb.rec072553 Cold Spring Harb Protoc 2012: pdb.rec072553- © 2012 Cold Spring Harbor Laboratory Press
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