Images of the mouse spinal cord. (A) Bright-field overview of the spinal cord. Note the pattern of dark blood vessels. (B) In vivo 2pLSM of green fluorescent microglia. The spinal capillaries are labeled with Texas Red-dextran by injection into the tail vein. (C) Recording from a double-transgenic mouse (TgH(CX3CR1-eGFP)) × (TgN(Thy1-eYFP)) with green fluorescent microglia and yellow fluorescent axons (shown in red). Note the oblique axons from the roots in contrast to the spinal axons running in parallel. (D) Recording from a double-transgenic (TgN(GFAP-eCFP)) × (TgN(Thy1-eYFP)) mouse. The thin lamellipodia of the cyan-fluorescent astrocytes are faint but clearly present. (E,F) Repetitive imaging of single spinal axons in TgN(Thy1-eGFP) mice. (E) A single axon was dissected by a high-power laser pulse. Laser lesion was carried out on a square field of 7 × 7 μm, laser power being set to 50% (1 W), for one image (i.e., ∼0.5 sec). (F) Three days later, the dissected axon shows some sprouting.