Recipe

Ablation Medium

Heat a paste of yeast and H2O in a microwave until boiling. When cool, it should have the consistency of maple syrup. (This yeast suspension is used as a control for ablation.) For complete ablation, make a suspension of 10–50 mg of hydroxyurea/mL of yeast paste at room temperature. For partial ablation, start with 3 mg of hydroxyurea/mL. Vary the dose in subsequent experiments, depending on the result. Mix the suspension continually. Prepare fresh on each day of treatment.

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  1. doi:10.1101/pdb.rec068049 Cold Spring Harb Protoc 2012: pdb.rec068049- © 2012 Cold Spring Harbor Laboratory Press
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