Provasoli-Enriched Seawater (PES)
| Reagent | Quantity (for 1 L) |
|---|---|
| Natural seawater | 1 L |
| Provasoli solution | 20 mL |
If possible, seawater should be collected by boat at some distance from the coast. Filter the seawater using a 5-μm mesh. Aliquot into Nalgene bottles (in glass bottles a precipitate can form), autoclave, and store at 13°C. Autoclave the filtered seawater and the Provasoli solution separately to avoid precipitation. This recipe is based on Starr and Zeikus (1993); we use half-strength PES (i.e., 10 mL of Provasoli solution per 1 L of autoclaved seawater).
- © 2012 Cold Spring Harbor Laboratory Press
