Recipe

Artificial Cerebrospinal Fluid (ACSF) for SR101 Labeling

125 mm NaCl

5 mm KCl

10 mm d-glucose

10 mm HEPES

2 mm MgSO4

2 mm CaCl2

Adjust pH to ∼7.3 with NaOH. Osmolarity should be ∼300.

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  1. doi:10.1101/pdb.rec068346 Cold Spring Harb Protoc 2012: pdb.rec068346- © 2012 Cold Spring Harbor Laboratory Press
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