Recipe

HL5 Medium for TIRFM

30.8 g glucose

14.3 g yeast extract

28.6 g Proteose Peptone No. 2 (Difco)

0.97 g KH2PO4

2.56 g Na2HPO4 • 12H2O

0.4 mg folic acid

0.12 mg cyanocobalamin

Dissolve the ingredients in 2 L of H2O. Sterilize by autoclaving.

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  1. doi:10.1101/pdb.rec068486 Cold Spring Harb Protoc 2012: pdb.rec068486- © 2012 Cold Spring Harbor Laboratory Press
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