Antibiotic Mix
| Reagent | Quantity (for 100 mL) |
|---|---|
| Penicillin G | 1 g |
| Streptomycin | 0.5 g |
| Chloramphenicol | 0.1 g |
Dissolve the penicillin G and streptomycin in 90 mL of water, then add the chloramphenicol dissolved in 10 mL of 100% ethanol. Filter through a 0.22-μm membrane and store in 5-mL aliquots at −20°C. Add 5 mL of the antibiotic mix to 1 L of Provasoli-enriched seawater (PES).
- © 2012 Cold Spring Harbor Laboratory Press
