In Vitro Transfection with Plasmid DNA Using Polylysine Copolymers
Adapted from Gene Transfer: Delivery and Expression of DNA and RNA (ed. Friedmann and Rossi). CSHL Press, Cold Spring Harbor, NY, USA, 2007.Abstract
The terplex system for delivering DNA to targeted cells comprises three components—stearyl polylysine (PLL), low-density lipoprotein (LDL), and DNA. This article describes a method for optimizing the relative amounts of these components and determining the transfection parameters for a given cell line.
- © 2012 Cold Spring Harbor Laboratory Press
