Recipe

G-Actin Buffer

2 mm Tris-Cl (pH 8.0 at 20°C)

0.2 mm CaCl2

0.2 mm ATP (added fresh)

0.5 mm dithiothreitol (DTT; prepared from powder daily)

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  1. doi:10.1101/pdb.rec069690 Cold Spring Harb Protoc 2012: pdb.rec069690- © 2012 Cold Spring Harbor Laboratory Press
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