Recipe

Motility Buffer for Actin

5–50 µm ATP

10 mm phosphocreatine (stocks stored at –20 °C and thawed daily; Sigma-Aldrich P7936)

0.3 mg/mL creatine phosphokinase (prepared daily from powder; Sigma-Aldrich C3755)

50 mm DTT (prepare 1 m DTT stock in M5+ daily)

Prepare daily in M5+.

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  1. doi:10.1101/pdb.rec070201 Cold Spring Harb Protoc 2012: pdb.rec070201- © 2012 Cold Spring Harbor Laboratory Press
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