Table 1.
Properties of the state-of-the-art GECIs in acute mouse brain slices
| ΔR/R, ΔF/Fa | Kineticsa | ||||
|---|---|---|---|---|---|
| Indicator | 1 AP | 40 APs | Rise time (msec) | Decay time (msec) | Photostabilityb |
| D3cpV | 5% ± 3% | 40% ± 20% | 100 ± 30 | 9500 ± 3400 | CFP: 59%; |
| YFP: 84% | |||||
| TN-XXL | 4% ± 2% | 50% ± 10% | 80 ± 20 | 1600 ± 600 | CFP: 36%; |
| YFP: 70% | |||||
| G-CaMP3 | 14.3% ± 3% | 500% ± 200% | 100 ± 30 | 650 ± 200 | GFP: 109% |
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From Tian et al. (2009).
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GECI, genetically encoded calcium indicator; AP, action potential.
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aMean ± s.d.
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bFluorescence after 30-min imaging.
