Recipe

Complete BHK Medium

4 mm glutamate

10% fetal calf serum (FCS)

Add glutamate and FCS to Iscove's modified Dulbecco's medium: Dulbecco's modified F-12 medium (GIBCO/Life Technologies) (1:1).

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  1. doi:10.1101/pdb.rec070789 Cold Spring Harb Protoc 2012: pdb.rec070789- © 2012 Cold Spring Harbor Laboratory Press

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