Hybridization Buffer (RNase-free)
| Reagent | Quantity (for 250 mL) | Final concentration |
|---|---|---|
| Formamide | 125 mL | 50% (v/v) |
| SSC (20× stock) | 62.5 mL | 5× |
| Herring sperm DNA (10 mg/mL), extracted twice with 1:1 phenol: chloroform | 2.5 mL | 100 mg/L |
| Heparin (50 mg/mL) | 250 µL | 50 mg/L |
| Tween 20 (10%, v/v) | 2.5 mL | 0.1% (v/v) |
| H2O (diethyl pyrocarbonate [DEPC]-treated) | 57.25 mL |
- © 2012 Cold Spring Harbor Laboratory Press
