Table 1.

Scaling up or down for different plate formats:

Multiply all volumes and cell numbers by the surface area of the desired plate divided by the surface area of the six-well plate.

Culture vessel 96 well 48 well 24 well 12 well 6 well 35 mm 60 mm 100 mm 150 mm T25 T75
Surface area (cm2) 0.3 0.7 2 4 10 10 20 60 140 25 75

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  1. Cold Spring Harb Protoc 2012: pdb.prot071076-