Recipe

MBS (100×)

88 mm NaCl

1 mm KCl

2.4 mm NaHCO3

10 mm HEPES

0.82 mm MgSO4•7H2O

0.33 mm Ca(NO3)2•4H2O

0.41 mm CaCl2•6H2O

Prepare in sterile H2O. Adjust the pH to 7.5.

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  1. doi:10.1101/pdb.rec070565 Cold Spring Harb Protoc 2012: pdb.rec070565- © 2012 Cold Spring Harbor Laboratory Press
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