Determining the Yield of RNA Synthesized In Vitro
Adapted from RNA: A Laboratory Manual, by Donald C. Rio, Manuel Ares Jr, Gregory J. Hannon, and Timothy W. Nilsen. CSHL Press, Cold Spring Harbor, NY, USA, 2011.Abstract
A simple way to determine the amount of RNA made by in vitro transcription is to include a trace amount of radioactive nucleotide and calculate the amount of nucleotides incorporated into the RNA, as described here. This protocol can also be used to quantitate unlabeled transcripts, assuming that the unlabeled RNA is equivalent to an aliquot of labeled RNA synthesized in parallel.
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