Kinase Buffer (10×)
| Reagent | Quantity (for 1 mL) | Final concentration (10×) |
|---|---|---|
| Tris–HCl (1 m, pH 7.5) | 500 µL | 500 mm |
| MgCl2 (1 m) | 100 µL | 100 mm |
| Dithiothreitol (DTT; 1 m) | 50 µL | 50 mm |
| H2O | 350 µL | – |
| Store for up to 6 mo at −20°C. | ||
- © 2013 Cold Spring Harbor Laboratory Press
