Ligation Buffer (2×)
| Reagent | Quantity (for 1 mL) | Final concentration (2×) |
|---|---|---|
| Tris–HCl (1 m, pH 7.6) | 132 µL | 132 mm |
| MgCl2 (1 m) | 20 µL | 20 mm |
| Dithiothreitol (DTT; 1 m) | 2 µL | 2 mm |
| ATP (100 mm) | 10 µL | 1 mm |
| Polyethylene glycol (PEG; MW 6000) (20%, w/v) | 750 µL | 15% (w/v) |
| H2O | 86 µL | – |
| Store for up to 6 mo at –20°C. | ||
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