Recipe

Pipette Solution (Containing NP-EGTA) for K+ Current Recordings

Reagent Final concentration
K glutamate 135 mm
HEPES 10 mm
NP-EGTA 10 mm
CaCl2 2 mm
Fluo-4 K+ salt 250 µm
Adjust pH to 7.2 with NMDG.
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  1. doi:10.1101/pdb.rec073379 Cold Spring Harb Protoc 2013: pdb.rec073379- © 2013 Cold Spring Harbor Laboratory Press
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