HEPES-Buffered Saline (HBS) (Used for Dye Loading)
| Reagent | Final concentration |
|---|---|
| NaCl | 135 mm |
| KCl | 5.9 mm |
| HEPES | 11.6 mm |
| CaCl2•2H2O | 1.5 mm |
| Glucose | 11.5 mm |
| MgCl2•6H2O | 1.2 mm |
| Adjust the pH to 7.3 with 5 m NaOH. | |
- © 2013 Cold Spring Harbor Laboratory Press
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doi:10.1101/pdb.rec075929 Cold Spring Harb Protoc 2013. 2013: pdb.rec075929- © 2013 Cold Spring Harbor Laboratory Press
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