Buffer D for Yeast Extracts
| Reagent | Quantity (for 2 L) | Final concentration |
|---|---|---|
| HEPES (1 m, pH 7.9 at 25°C) | 20 mL | 20 mm |
| KCl (3 m) | 33.3 mL | 50 mm |
| EDTA (0.5 m, pH 8.0) | 0.8 mL | 0.2 mm |
| Glycerol (100%, v/v) | 400 mL | 20% (v/v) |
| H2O | to 2 L | |
| Store at 4°C. | ||
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