T7-RNAP Storage Buffer
| Reagent | Quantity (for 1 L) | Final concentration |
|---|---|---|
| Potassium phosphate (0.2 m, pH 7.5) | 100 mL | 20 mm |
| NaCl (5 m) | 20 mL | 100 mm |
| Glycerol (100%) | 500 mL | 50% (v/v) |
| Dithiothreitol (DTT, 0.5 m) | 20 mL | 10 mm |
| EDTA (0.25 m, pH 8.0) | 0.4 mL | 0.1 mm |
| NaN3 powder (Sigma-Aldrich) | 2 g | 0.2% (w/v) |
| ddH2O | to 1 L |
- © 2013 Cold Spring Harbor Laboratory Press
